Nature Methods 12, 1015 (2015). doi:10.1038/nmeth.3625

Author: Vivien Marx

It's been a long wait for nanopore sequencing technology. Over 1,000 labs are testing the first commercial device and publishing results. Researchers tell Nature Methods about their experiences putting these early instruments through their paces. And more technology is in the works.

Nature Methods 12, 1019 (2015). doi:10.1038/nmeth.3641

Author: David Baddeley

Two approaches for robust, objective segmentation of single-molecule localization data promise better quantitative insights into protein clustering from super-resolution imaging methods.

Nature Methods 12, 1021 (2015). doi:10.1038/nmeth.3623

Authors: Kedar Narayan & Sriram Subramaniam

Nature Methods 12, 1098 (2015). doi:10.1038/nmeth1115-1098a

Author: Che-Yang Liao, Wouter Smet, Geraldine Brunoud, Saiko Yoshida, Teva Vernoux & Dolf Weijers

Nature Methods 12, 1098 (2015). doi:10.1038/nmeth1115-1098b

Author: Jorge López Puga, Martin Krzywinski & Naomi Altman

Nature Methods 12, 1098 (2015). doi:10.1038/nmeth1115-1098c

Author: Gagarine Yaikhom, Hugh Morgan, Duncan Sneddon, Ahmad Retha, Julian Atienza-Herrero, Andrew Blake, James Brown, Armida Di Fenza, Tanja Fiegel, Neil Horner, Natalie Ring, Luis Santos, Henrik Westerberg, Steve D M Brown & Ann-Marie Mallon

Nature Methods 12, 1098 (2015). doi:10.1038/nmeth1115-1098d

Author: Matthew D W Piper, Eric Blanc, Ricardo Leitão-Gonçalves, Mingyao Yang, Xiaoli He, Nancy J Linford, Matthew P Hoddinott, Corinna Hopfen, George A Soultoukis, Christine Niemeyer, Fiona Kerr, Scott D Pletcher, Carlos Ribeiro & Linda Partridge

Nature Methods 12, (2015). doi:10.1038/nmeth.f.384

Authors: Steven Thomas, Nathaniel D Maynard & John Gill

Nature Methods 12, (2015). doi:10.1038/nmeth.f.385

Author: Lynne Chang

Nature Methods 12, (2015). doi:10.1038/nmeth.f.386

Authors: Cassie Schumacher, Laurie Kurihara & Kate Cunningham

Nature Methods 12, (2015). doi:10.1038/nmeth.f.387

Authors: Philip Lee, Cindy S Y Chen, Terry Gaige & Paul J Hung

Nature Methods 12, 1072 (2015). doi:10.1038/nmeth.3612

Authors: Patrick Rubin-Delanchy, Garth L Burn, Juliette Griffié, David J Williamson, Nicholas A Heard, Andrew P Cope & Dylan M Owen

Nature Methods 12, 895 (2015). doi:10.1038/nmeth.3618

Some cells have a remarkable capacity to organize into tissue-like structures in vitro. As methods to enable self-organization improve, ethical aspects of some of these experiments will need to be considered.

Nature Methods 12, 897 (2015). doi:10.1038/nmeth.3592

Author: Vivien Marx

Creative chemical techniques to better understand tissue self-organization.

Nature Methods 12, 899 (2015). doi:10.1038/nmeth.3587

Authors: Naomi Altman & Martin Krzywinski

Nature Methods 12, 901 (2015). doi:10.1038/nmeth.3588

Authors: Gayan Senavirathne, Jiaquan Liu, Miguel A Lopez, Jeungphill Hanne, Juana Martin-Lopez, Jong-Bong Lee, Kristine E Yoder & Richard Fishel

Nature Methods 12, 902 (2015). doi:10.1038/nmeth.3589

Authors: Duy Tin Truong, Eric A Franzosa, Timothy L Tickle, Matthias Scholz, George Weingart, Edoardo Pasolli, Adrian Tett, Curtis Huttenhower & Nicola Segata

Nature Methods 12, 905 (2015). doi:10.1038/nmeth.3605

Author: Nina Vogt

Developments in automated sample preparation, electron microscopy and data analysis enable in-depth characterization of a chunk of mouse neocortex.

Nature Methods 12, 906 (2015). doi:10.1038/nmeth.3606

Author: Rita Strack

Proximity ligation allows for the global resolution of RNA secondary structures.

Nature Methods 12, 906 (2015). doi:10.1038/nmeth.3607

Author: Allison Doerr

Researchers use chemistry to solve a nagging problem that has challenged the analysis of RNA from formaldehyde-fixed tissue specimens.