Nature Methods

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Nature Methods is a science methodology journal publishing laboratory techniques and methods papers in the life sciences and areas of chemistry relevant to the life sciences.
Updated: 8 years 17 weeks ago

Microbiology: Keeping track of gut bacteria

Mon, 09/28/2015 - 23:00

Nature Methods 12, 908 (2015). doi:10.1038/nmeth.3608

Author: Irene Jarchum

Fluorescent labeling of bacteria enables tracking of their location.

Cell biology: High-throughput discovery of genome organizers

Mon, 09/28/2015 - 23:00

Nature Methods 12, 909 (2015). doi:10.1038/nmeth.3598

Genomics: Variation in human repeat sequences

Mon, 09/28/2015 - 23:00

Nature Methods 12, 909 (2015). doi:10.1038/nmeth.3599

Stem cells: Zapping cells improves genome-editing efficiency

Mon, 09/28/2015 - 23:00

Nature Methods 12, 909 (2015). doi:10.1038/nmeth.3600

Microscopy: Isotropic super-resolution imaging

Mon, 09/28/2015 - 23:00

Nature Methods 12, 909 (2015). doi:10.1038/nmeth.3601

Model organisms: Expanding GFP utility beyond imaging

Mon, 09/28/2015 - 23:00

Nature Methods 12, 910 (2015). doi:10.1038/nmeth.3594

Bioinformatics: How good are those RNA-seq data?

Mon, 09/28/2015 - 23:00

Nature Methods 12, 910 (2015). doi:10.1038/nmeth.3595

Microbiology: Ancestral AAVs put to new use

Mon, 09/28/2015 - 23:00

Nature Methods 12, 910 (2015). doi:10.1038/nmeth.3596

Molecular engineering: A suite of oligomeric GFP variants

Mon, 09/28/2015 - 23:00

Nature Methods 12, 910 (2015). doi:10.1038/nmeth.3597

Bioinformatics: Finding a face in the crowd

Mon, 09/28/2015 - 23:00

Nature Methods 12, 911 (2015). doi:10.1038/nmeth.3609

Author: Michael Eisenstein

An analytical algorithm for transcriptomic data reveals rare cell types that were previously hidden in larger populations.

Molecular biology: Inseparable ribosomes

Mon, 09/28/2015 - 23:00

Nature Methods 12, 913 (2015). doi:10.1038/nmeth.3610

Author: Nicole Rusk

Fusing ribosomal subunits provides answers to basic questions about their function and opens the door to exciting engineering possibilities.

Stem cells: Fast track to neurons

Mon, 09/28/2015 - 23:00

Nature Methods 12, 915 (2015). doi:10.1038/nmeth.3611

Author: Tal Nawy

New protocols generate neurons directly from fibroblasts using only chemical cocktails.

What if stem cells turn into embryos in a dish?

Mon, 09/28/2015 - 23:00

Nature Methods 12, 917 (2015). doi:10.1038/nmeth.3586

Authors: Martin F Pera, Guido de Wert, Wybo Dondorp, Robin Lovell-Badge, Christine L Mummery, Megan Munsie & Patrick P Tam

Voltage sensors: challenging, but with potential

Mon, 09/28/2015 - 23:00

Nature Methods 12, 921 (2015). doi:10.1038/nmeth.3591

Author: Nina Vogt

Genetically encoded voltage sensors are coming of age, but their use still poses challenges that must be addressed from multiple angles.

Diving deeper to predict noncoding sequence function

Mon, 09/28/2015 - 23:00

Nature Methods 12, 925 (2015). doi:10.1038/nmeth.3604

Authors: Barbara E Engelhardt & Christopher D Brown

The deep learning–based method DeepSEA predicts the molecular function of noncoding DNA polymorphisms.

Single Molecule Cluster Analysis dissects splicing pathway conformational dynamics

Sun, 09/27/2015 - 23:00

Nature Methods 12, 1077 (2015). doi:10.1038/nmeth.3602

Authors: Mario R Blanco, Joshua S Martin, Matthew L Kahlscheuer, Ramya Krishnan, John Abelson, Alain Laederach & Nils G Walter

Comparing the performance of biomedical clustering methods

Sun, 09/20/2015 - 23:00

Nature Methods 12, 1033 (2015). doi:10.1038/nmeth.3583

Authors: Christian Wiwie, Jan Baumbach & Richard Röttger

Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling

Sun, 09/20/2015 - 23:00

Nature Methods 12, 1055 (2015). doi:10.1038/nmeth.3590

Authors: Kilian V M Huber, Karin M Olek, André C Müller, Chris Soon Heng Tan, Keiryn L Bennett, Jacques Colinge & Giulio Superti-Furga

Thermal stabilization of proteins after ligand binding provides an efficient means to assess the binding of small molecules to proteins. We show here that in combination with quantitative mass spectrometry, the approach allows for the systematic survey of protein engagement by cellular metabolites and drugs. We profiled the targets of the drugs methotrexate and (S)-crizotinib and the metabolite 2′3′-cGAMP in intact cells and identified the 2′3′-cGAMP cognate transmembrane receptor STING, involved in immune signaling.

DNA sense-and-respond protein modules for mammalian cells

Sun, 09/20/2015 - 23:00

Nature Methods 12, 1085 (2015). doi:10.1038/nmeth.3585

Authors: Shimyn Slomovic & James J Collins

Whole-brain activity mapping onto a zebrafish brain atlas

Sun, 09/13/2015 - 23:00

Nature Methods 12, 1039 (2015). doi:10.1038/nmeth.3581

Authors: Owen Randlett, Caroline L Wee, Eva A Naumann, Onyeka Nnaemeka, David Schoppik, James E Fitzgerald, Ruben Portugues, Alix M B Lacoste, Clemens Riegler, Florian Engert & Alexander F Schier